course structure
| units | topics | marks |
|---|---|---|
| v | protein & gene manipulation | 40 |
| vi | cell culture & genetic manipulation | 30 |
| practical | 30 | |
| total | 100 | |
course syllabus
unit v: protein and gene manipulation
chapter 1: recombinant dna technology
- introduction
- tool of rdna technology
- making rdna
- introduction of recombinant dna into host cells
- identification of recombinants
- polymerase chain reaction (pcr)
- hybridization techniques
- dna library
- dna sequencing
- site-directed mutagenesis
chapter 2: protein structure and engineering
- introduction to the world of proteins
- 3-d shape of proteins
- structure-function relationship in protein
- purification of proteins
- characterization of proteins
- protein based products
- designing proteins (protein engineering)
chapter 3: genomics and bioinformatics
- introduction
- genome sequencing projects
- gene prediction and counting
- genome similarity
- snps and comparative genomics
- functional genomics
- proteomics
- history of bioinformatics
- sequences and nomenclature
- information sources
- analysis using bioinformatics tools
unit vi: cell culture and genetic manipulation
chapter i: microbial culture and applications
- introduction
- microbial culture techniques
- measurement and kinetics of microbial growth
- scale up of microbial process
- isolation of microbial products
- strain isolation and improvement
- applications of microbial culture technology
- biosafety issues in microbial technology
chapter ii: plant cell culture and applications
- introduction
- cell and tissue culture techniques
- applications of cell and tissue culture
- gene transfer methods in plants
- transgenic plants with beneficial traits
- biosafety in plant genetic engineering
chapter iii: animal cell culture and applications
- introduction
- animal cell culture techniques
- characterisation of cell lines
- methods of gene delivery into cells
- scale-up of animal culture process
- applications of animal cell culture
- stem cell technology
- tissue engineering
practical works
list of experiments
isolation of bacterial plasmid dna and its detection by gel electrophoresis
restriction digestion of plasmid dna and its analysis by gel electrophoresis
bacterial transformation using any plasmid
data retrieval and data base search using internet site ncbi
download a dna and protein sequence from internet, analyse it and comment on it
cell viability assay
determination of blood groups
estimation of dna
ion-exchange chromatography for proteins
reading of dna sequencing gel to arrive at the sequence
estimation of blood glucose by enzymatic method (god/pod)
project work
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